Copper Metabolism Lab @ IISERK
Summary diagram showing pathways of copper dependent ATP7B trafficking in control polarized WIF-B cells (left) and cells overexpressing MyoVbT (right). Our data suggest that ATP7B is transported to the apical luminal surface through a process that requires MyoVB. Overexpression of the MyoVbT (red) arrests ATP7B (green) in a subapical vesicular compartment (brown)
Gupta et al J Cell Sci. 2016;129(6):1179-89.
Low copper (Cu) promotes ATP7B-Arg875 exit from the ER. Costaining with anti-Flag (green) and TGN38 (red) antibodies was performed following overnight incubation of Hek293Trex cells with 0–10 μM copper. At 2 μM copper, ATP7B-Arg875 is found predominantly in the TGN, whereas at 10 μM copper, additional vesicular localization (arrows) becomes apparent.
Gupta et al Proc Natl Acad Sci U S A. 2011 ;108(13):5390-5